All of my Primers | COLLECTION SERIES (2023)


Products mentioned in the video:
Essence Hello Glow Serum Primer:
Elf Power Grip Primer:
Collection Filter Finish:
PS... Flawless Glow:
PS... Prep & Perfect Vitamin-Enriched Moisturising Primer:


Foreign series videos so I'm going through my collection and I'm talking more depth about products because I can't and I, just think it's better to get a little a little snippet review when you're looking at these products.

So today, I've already done Foundation, concealer and I'm, backtracking and going for primer I own five primers, which isn't a lot of primers, but it's a lot for me to be honest.

It is a lot of primers for me.

So yeah, going into the one that I'm using the most recently it's, the essence, hello.

Good stuff.


Serum primer.

This is supposed to meet you for the glow recipe.

One never tried.

It I do love this though I love the pump I've used a good chocolate.

Soaker, I, really like the smell of this I think it's.

It is glowy.

It is glowy.

It is very glowy and I have dry skin.

So it is very glowy I, don't know, if you if you had oily skin, would you like it as much because it's radiant, but um, I, really like it.

The smell is nice.

It pairs really well with kind of more matte foundations for me because, uh, it gives them matte foundations more of a glow, which I enjoy I like a bit of a glow.

You see me she me, yeah, I like the glowiness and I really enjoy.

This I think for the price of it.

The amount you get you get 30 ml of it.


So you get 1.01.

Fluid ounces, I think standard add a little bit in a bigger bottle to be quite honest, but I think all of these are the same and I I, really like this I love.

The pump I would reuse, the glass bottle, I, don't know what for but that's, just the ordering me I want to reuse the bottle.

Next one I have is the collection filter, finish complexion, boosting, primer and Illuminator.

This is the second one of these that I own I, get it in the shade Fair.

This is beautiful it's, very some people would say it, metallic some people really don't like this it's supposed to be a Jeep for the I think it's Hollywood closed.


It is supposed to read you for that.

This is way more metallic or glowy, um, which I prefer under my foundations I.

Think it looks beautiful I, really think it makes your finishings glow.

Some people don't like it, because the people that I paint that pick up use the Hollywood Flawless filter more as a foundation on its own.

And this is not a product to wear on its own.

This is a primer or kind of a cream highlight, I think, it's, really nice.

It looks darker I haven't used this in a while because I weaned myself, also because I'm using it so much, um, yeah, I was like I need to try other things or I will literally die of my pores.

Just rejecting this because I wear it so much.

But um, it's, really nice at 7.99 in Boots and I, don't know where else you can buy collection and Superdrug I've, never seen in Super, Truck, it's, always sole deck.

Because it is a very nice product and I would recommend that I've used it up I've recommended this to a point where my future, mother-in-law auntie-in-law and granny and little have bought it.

And it was really good because the time when they decided to buy it was three for two and boots and I was like I want to use getting this for free.

But uh, yeah, I really I, really like it.

I think it's, really nice.


Why I'm is she I've? I, I'm ashamed.

So by popular, um, online, Trend, I, got the elf.

Paragraph primer I, haven't, used it a lot.

Uh since I bought it I've used it once or twice and I think it's, fine I haven't had the like like stickiness, I, don't know, if I'm using the right amount of it, but I can't, wait for the summer to use this when things are literally sliding off my face.

So I, can't, wait for that.

But I've heard really good pings on the new ones because my I've heard it's better for oily skin.

So I got this one.

It said, the other one, yeah, I have no opinion on this one I'm going on to my pennies makeup.

Oh, this is the PS prep and perfect.

Vitamin enriched moisturizing primer.

This is 1.5, fluid ounces, it's supposed to be a Jeep for the Bobby Brown.

One I have not bought a Bobby Brown because I can play it over I.

Think my most expensive primer was close to the tenor pin over tenor of crazy.

Girl, crazy, um.

But uh, this is really nice.

This is very moisturizing, it's, I feel like people who Skip moisturizing, their skin would use this and be like this is beautiful.

This is beauty.

This is gray.

So this is going all over my face, which I really enjoy this I think, uh, when I'm really lazy and I just get moisturizer.

So I just SPF.

My face I, throw this on and I'm like moisture and it's, really nice.

It smells.

Good smells like a spa.

But I think this is really nice.

It has old oil shea butter and contains vitamin E C and my cinnamon.

Now when they put vitamin E into a vitamin when they put like vitamin E into stuff, I'm, just like my Skin's, not gonna absorb that at all the reason I'm, not too sure in vitamin C I do use the vitamin C serum.

So I hope it does something and my son I might I hope.

It does something but I don't know and it's made in Thailand.

Pretty cool.

Pretty cool.

I do recommend this it's like 450 or Fiber, which for the amount you get it's such a nice tub.

Why not this is always sold out though I got this on a fluke one day, I really have to be on the ball and checking Penny's like every day or every week.

And the other thing I have is the PS Flawless.

Yep, Flawless glow, radiant glow, primer hybrid, primer Foundation hybrid.

And this is Penny's version of the Hollywood Flawless filter, which as you can see that just looks like delicious, oiliness doesn't.

It I get it in the shade buttermilk.

This is Radiance.

This is glowy.

This is I mix like a shimmer into my Foundation.

I know, my skin has a healthy glow.

Uh, I.

Do like this I don't like it as much as my collection one, though so I'd say this one is closer to the shower Tilbury.

One, actually it is.

It is closer to the channel to everyone I don't like the shower to everyone to be Frank, um it's, too nothing to me.

And so this is very nothing to me.

Um I do like the doe foot and the sound like ASMR, a ASM or for the win for a yeah.

So it's, a Euro cheaper than the collection one.

So if you don't like the collection one because it's not similar enough to the shower to everyone grab the one and pennies, if you can find your shade like a buttermilk, which I think is the lightest.

And this is the teeniest book you like for me, um, but yeah, that's.

What it is I have no big opinions on primers, I know, if they don't work for my face as you can see they're all very glowy, radiant supporting kind of primers, I, don't have any mattifying primers, I, don't, see the point of modifying primers, um, because most of the time they are just full of silicon that is just trying to make the pores.

Look like they're, not there which I don't care.

And if you want to see any other part of my collection, I haven't done bronzer, blush lipsticks lip liners eyeliners, eyeshadows, I, haven't done those.

If you want to see those, let me know, um, I'm, just gonna take a break for a while from doing the bases well, I haven't done my patterns either, um or setting sprays.


Thank you so much for watching if you want to see more from me, please subscribe like and I'll catch you later, bye.


How many sets of primers are needed? ›

In any cycle of polymerase chain reaction , two sets of primers and the enzyme DNA polymerase are needed. > Role of primers and DNA polymerase: To begin the functioning of DNA polymerase, primers are needed.

How many primers for sequencing? ›

A Sanger sequencing reaction is run with a single primer.

Why does my PCR keep failing? ›

Insufficient amplification can result if the initial amount of template is too low. Increase the number of amplification cycles in increments of 5, or, if possible, increase the amount of template. Contaminants in the dNTP mix can lead to incomplete or incorrect amplification or PCR inhibition. Use high-quality dNTPs.

How many primers do you need for PCR? ›

The PCR reaction uses two primers complementary to and hybridizing with opposite strands of the DNA with one to the left (5′) and one to the right (3′) of the target sequence to be amplified.

How do I choose a primer sequence? ›

Here are a few things to keep in mind when designing your own primers.
  1. Primer length should be in the range of 18 to 22 bases.
  2. The primer should have GC content of 50% to 55%.
  3. Primers should have a GC-lock on the 3' end.
  4. The melting temperature of any good primer should be in the range of 50OC to 55OC.
Dec 8, 2015

Are shorter or longer primers better? ›

The shorter the primers, the more efficiently they can anneal to target DNA. Primers that are longer—say 28 to 35 bases—work better when troubleshooting closely related species, for instance. The longer range allows for higher specificity and room for adding restriction enzyme sites to the primer end, if cloning.

What strand needs multiple primers? ›

For this reason, multiple primers need to be formed on the lagging strand so DNA polymerase can synthesize in the 5' to 3' direction from every primer. These fragments of DNA are called Okazaki fragments.

Do you need two primers for sequencing? ›

Sequencing uses only one primer instead of the two used in PCR. If you do not remove both primers, you will get two sequences superimposed on each other that are not readable.

How do you count primers? ›

Primer information sent from a company will list the molecular weight of your primer (g/mol) and the amount of nmoles that they sent. If you have 29.4 nmol of primer and you want to make a 100 μM stock solution in TE – simply multiply the amount of nmoles by a factor of ten and add that many microliters.

What are the common mistakes in PCR? ›

Many of the common problems with PCR and RT-PCR are identified during agarose gel electrophoresis of the reaction products. These include the absence of the expected amplification product, the presence of nonspecific products, excessive smearing, and the presence of a “primer dimer” band.

Why are my primers not working? ›

Too high annealing temperature used

For your primers to successfully bind to your template DNA they require an optimum annealing temperature during the annealing phase of the PCR reaction. Using too high of an annealing temperature will prevent your primers from binding to the complementary DNA.

What are 2 possible reasons for a unsuccessful PCR run? ›

The reason for PCR failure is usually rapidly identified. Often problems can be explained by the fact that essential components like Mg2+ ions or even primers were of poor quality because the expiration date has passed or were unintentionally not added to the reaction mix.

Can you do PCR with only one primer? ›

If only one primer is used, the process is called “asymmetric PCR”. Only one strand of the double-stranded DNA will be amplified, and only one new copy is synthesized per cycle, which is unable to achieve exponential amplification.

Can you put more than 2 primers in PCR? ›

In multiplex PCR more than one target sequence can be amplified by including more than one pair of primers in the reaction.

Why only 2 primers are needed for PCR? ›

Because DNA polymerase can add a nucleotide only onto a preexisting 3'-OH group, it needs a primer to which it can add the first nucleotide. This requirement makes it possible to delineate a specific region of template sequence that the researcher wants to amplify.

How do you know which primers to use in PCR? ›

What makes a good primer?
  1. Aim for the GC content to be between 40 and 60% with the 3' of a primer ending in G or C to promote binding. ...
  2. A good length for PCR primers is generally around 18-30 bases. ...
  3. Try to make the melting temperature (Tm) of the primers between 65°C and 75°C, and within 5°C of each other.
Sep 25, 2019

Do primers have to match perfectly? ›

However, primers do not need to correspond to the template strand completely; it is essential, however, that the 3' end of the primer corresponds completely to the template DNA strand so elongation can proceed.

Which primer goes first? ›

So, next time you're wondering about application order, remember that your moisturizer comes before your primer and soon, it will become a natural part of your skin care routine.

How far apart should PCR primers be? ›

Forward and reverse primers should be about 500 bp apart. The 3' end of the primer should be a G or a C. The genomic sequence that comes from the computer is just one strand; the complementary strand is not shown. For the forward primer, you can use the sequence directly.

Is it OK to use multiple primers? ›

Primers are built for layering, so if you have multiple skin concerns, feel free to layer different primers. Based on your skin type (oily, normal or dry), the right primer will smooth over fine lines and wrinkles, seal pores and increase the longevity of foundation—every makeup lover's dream.

What are the 3 main strategies for primer design? ›

There are 3 strategies for primer design: 1) insert-specific primers, 2) backbone-specific primers, and 3) orientation-specific primers.

Are primers always 5 to 3? ›

About primers and their direction in PCR:

Since DNA synthesis is always from 5' to 3' , the 3' ends of a PCR primer set point towards each other, when they are annealed to their template strand, and the primers anneal on opposite strands of the PCR template.

Can you do PCR with 3 primers? ›

This technique, called RT-triple primer-PCR, consists of coamplification of wild-type and truncated cDNAs using three primers in the PCR. To validate this approach, a truncated estrogen receptor variant (clone 4) was quantified relative to the wild-type estrogen receptor using plasmid preparations.

Does lagging strand require more primers? ›

The leading strand can be extended from one primer alone, whereas the lagging strand needs a new primer for each of the short Okazaki fragments.

Do primers have to exactly match DNA? ›

The 3' end of the primer should be an exact match to the template DNA, because extension by DNA polymerase, during PCR, depends on a good match at the 3' end.

Why does DNA sequencing only need 1 primer? ›

Only one primer is used in DNA sequencing as the use of two primers would result in conflicting information from the sequencing. By sequencing only one strand of DNA we can determine the order of the nucleotides on that strand and, by inference, determine the sequence of nucleotides on the second stand.

Why would you only include 1 primer in a sequencing reaction instead of 2? ›

Because only one primer is used, only one strand is copied during sequencing, there is a linear increase of the number of copies of one strand of the gene.

How many sets of primers are needed for DNA profiling? ›

Typically, between 10-15 sets of primers are used to amplify different regions of the genome in order to generate a unique DNA profile.

What concentration should I order primers? ›

First, identify the concentration required for your working stock. For primers, this typically ranges from 10–100 µM, and for probes, from 2–10 µM.

How many base pairs can a primer sequence? ›

Range for primers should be at least 50-100 bp in length for both forward and reverse primers.

How do I make sure my PCR can run well? ›

13 Easy Tips for Avoiding PCR Errors
  1. 1 – Set Up a Sterile Environment. ...
  2. 2 – Check template purity and concentration. ...
  3. 3 – Take inventory of aliquoted PCR reagents. ...
  4. 4 – Make sure you choose the right annealing temperature. ...
  5. 5 – Avoid overloading your wells with product. ...
  6. 6 – Check off each reagent as it's added to the master mix.
Jul 9, 2020

Why are my PCR bands faint? ›

If you see faint or no bands on the gel:

There was insufficient quantity or concentration of DNA loaded on the gel. Increase the amount of DNA, but don't exceed 50 ng/band. The DNA was degraded. Avoid nuclease contamination.

How to improve PCR yield? ›

Hot-start DNA polymerases also increase yields of the desired PCR products by eliminating nonspecific amplification. Alternatively, set up PCR on ice, or add DNA polymerase last to the reaction mixture.

Why am i getting multiple bands in PCR? ›

The unexpected or multiple bands that you are experiencing in your PCR results, is most likely the result of nonspecific binding or the formation of a primer-dimer.

How do I know if my primer is correct? ›

Choose a primer set with minimum secondary structure formation. Thereafter can use Primer BLAST for the primers obtained using IDT oligo analyser. If the blast result shows the the genetic sequence of your desired gene, the primers designed are perfect.

What happens if you add too much primer to a PCR? ›

The amount of DNA template in a PCR has a negative effect on the outcome of a PCR procedure. Using too much DNA template, results in packed DNA in the confined space of the reaction vessel and can lead to false priming and even poor DNA synthesis due to the obstructed diffusion of large Taq polymerase molecules.

How do you know if primers are degraded? ›

At present, the most reliable method of quality assurance is mass spectrometry. You can see clearly how many base deletions and mismatches are found in your primers.

What if annealing temperature is too low for primer? ›

If temperature is too high the primers cannot anneal efficiently, and if the annealing temperature is too low the primers may bind nonspecifically to the template. They are present in large excess, so this step can be repeated a lot of times.

Why did my PCR smear? ›

Excessively long extension times may result in smearing. The general recommendation for extension time for this enzyme is 10–20 sec/kb. If PCR yield is low, try increasing the number of cycles by 5.

Why are 2 sets of primers required for PCR to work? ›

The use of two pairs of oligonucleotides allows a higher number of cycles to be performed, thereby increasing the sensitivity of the PCR. The improved specificity of the reaction derives from the binding of two separate sets of primers to the same target template.

How many RNA primers are needed? ›

Answer and Explanation: Only one RNA primer is required on the leading strand, and DNA is produced continuously, whereas DNA is created in small lengths on the lagging strand, each of which must begin with its RNA primer. For the initiation and spread of leading strand synthesis, only one primer is required.

How many primers does this strand need to replicate? ›

Only one primer is required for the initiation and propagation of leading strand synthesis. Lagging strand synthesis is much more complex and involves five steps. 1.

Can you do PCR with one primer only? ›

If only one primer is used, the process is called “asymmetric PCR”. Only one strand of the double-stranded DNA will be amplified, and only one new copy is synthesized per cycle, which is unable to achieve exponential amplification.

Can you use two primers at once PCR? ›

Two primers are used in each PCR reaction, and they are designed so that they flank the target region (region that should be copied). That is, they are given sequences that will make them bind to opposite strands of the template DNA, just at the edges of the region to be copied.

Can you use multiple primers in PCR? ›

In multiplex PCR more than one target sequence can be amplified by including more than one pair of primers in the reaction. Multiplex PCR has the potential to produce considerable savings of time and effort within the laboratory without compromising test utility.

How much primer for DNA for PCR? ›

In setting up PCR, primers are added to the reaction in the range of 0.1–1 μM. For primers with degenerate bases or those used in long PCR, primer concentrations of 0.3–1 μM are often favorable. A general recommendation is to start with standard concentrations and adjust as necessary.

How many primers are used in PCR quizlet? ›

For a PCR reaction, two primers are used because: DNA is double-stranded. DNA polymerase synthesizes DNA in the 5'→3' direction. The forward primer anneals to one template strand, the reverse primer anneals to the other template strand, and both initiate DNA polymerase reactions in the correct direction.

Which 2 primers will copy the entire sequence of DNA? ›

To amplify any DNA sequence, two primers are necessary. One is called 'forward primer' and the other one is called 'reverse primer'. The forward primer synthesizes the upper strand using the bottom strand as a template. Whereas Reverse primer uses the upper strand as a template and synthesizes the lower strand.

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