Vivien C.
asked 10/21/20What are some errors that could occur when making a standard dilution and when plotting a calibration curve?
What about errors using a spectrophotometer?
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1 Expert Answer
By:
J.R. S. answered 10/22/20
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5.0 (145)
Ph.D. University Professor with 10+ years Tutoring Experience
The most common error made in standard dilutions is pipetting errors. Other errors include calculation errors and bad technique in transferring solutions from place to place.
Errors in "plotting" a calibration curve arise from errors in making the dilutions and not in the actual "plotting" of the curve. This is an ambiguous question.
Errors using a spectrophotometer may arise from an incorrect "blank", using unclean equipment (cuvettes, etc.), using an incorrect wavelength, etc.
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