A serial dilution is the stepwise dilution of a substance in a solution. Serial dilutions result in the geometric progression of the concentration in a logarithmic fashion, meaning the concentration decreases by the same quantity in each successive step. When performing a serial dilution remember to:
Keep the dilution factor (DF) for each step constant
For example, creating a four-fold dilution (DF = 1/4) with a starting concentration of 10 µM yields the following concentrations: 10 µM, 2.5 µM, 0.625 µM, 0.156 µM, 0.039 µM etc.
A serial dilution is a step-wise series of dilutions, where the dilution factor stays the same for each step. The purpose of a serial dilution is to estimate the concentration of a sample, or to obtain the desired concentration of a reagent, chemical or compound.
A serial dilution is a set of dilutions of a stock standard, stock reagent, or body fluid specimen that is prepared by utilizing each dilution as the starting solution for the next dilution. Each tube in a serial dilution contains an individual dilution and a net dilution.
A Serial dilution is a series of dilutions, with the dilution factor staying the same for each step. The concentration factor is the initial volume divided by the final solution volume. The dilution factor is the inverse of the concentration factor.
Serial dilution and direct dilution both yield a range of concentrations. However, the key distinction lies in their methods: Serial Dilution Series: Each concentration is created by diluting the previous one. This approach allows for accurate dilutions across a broader concentration range.
How much initial sample and diluent should you use? Answer: 1:5 dilution = 1/5 dilution = 1 part sample and 4 parts diluent in a total of 5 parts. If you need 10 ml, final volume, then you need 1/5 of 10 ml = 2 ml sample. To bring this 2 ml sample up to a total volume of 10 ml, you must add 10 ml - 2 ml = 8 ml diluent.
Serial dilution has many advantages: the materials necessary are typically already present in the lab and require no special engineering. Conditions can be adjusted as the experiment progresses (e.g., drug concentrations increased as drug resistance improves).
A ten-fold dilution reduces the concentration of a solution or a suspension of virus by a factor of ten that is to one-tenth the original concentration. A series of ten-fold dilutions is described as ten-fold serial dilutions.
The source of dilution material for each step comes from the diluted material of the previous. In a serial dilution the total dilution factor at any point is the product of the individual dilution factors in each step up to it. Final dilution factor (DF) = DF1 * DF2 * DF3, etc. 1:100 * 100 = 1:10,000.
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