What is an 'X' stock solution? (2024)

Posted August 5, 2019


Answer

The 'X' is used to denote the strength of the stock solution.

Stock solutions are routinely prepared as a concentrated solution, from which one can dilute into a normal working concentration for actual use. Using stock solutions are beneficial because they:

  • Save time
  • Conserve materials
  • Reduce storage space
  • Improve accuracy

Most applications require a normalworking concentration of 1X. For stock solutions with a higher concentration, dilute to 1X before using.

For example: A stock solution that is 500 times more concentration would be written as 500X and would require a 1:500 dilution to restore the normal working concentration. Therefore, to make a 1X working solution you would need to add 2 µL of your stock solution into 1 mL of a buffer of your choice.

What is an 'X' stock solution? (2024)

FAQs

What is an 'X' stock solution? ›

“X” solutions indicate relative concentration instead of absolute concentration. In other words, an “X” solution does not indicate the amount per unit volume, but rather how many times more concentrated a solution is than the final or desired concentration.

What does 500X solution mean? ›

For example: A stock solution that is 500 times more concentration would be written as 500X and would require a 1:500 dilution to restore the normal working concentration.

What does 20x stock solution mean? ›

Concentrated stock solutions - using "X" units

A solution 20 times more concentrated would be denoted as 20x and would require a 1:20 dilution to restore the typical working concentration. Example: A 1x solution of a compound has a molar concentration of 0.05 M for its typical use in a lab procedure.

What does 10X stock solution mean? ›

10X means the solution is ten times higher in the solute concentration. Example: If i have a solution of sugar with initial concentration of 1mg/ml and someone ask me to prepare a 10X solution. I simply add 10 times sugar (solute) to the existing amount of solvent.

What is an example of a stock solution? ›

Most commonly 10X (relative concentration) stock solutions are made to avoid complicated calculations. For example, you would add 100 grams of your solute to 1000mL water to make the 10% stock solution.

How to make 500X dilution? ›

Prepare a 500X dilution: Take 1 μL of the 100X dilution and add it to a total volume of 100 μL. This will yield a 500X dilution.

What is a 100x stock solution? ›

100x means that this solution is 100 -fold more concentrated than the working solution that you are going to used in your experiment. The working solution of Reagent A must be prepared by diluting in distilled water right before starting the experiment.

How to make 20x dilution? ›

A 20-fold dilution just means the final solution is 20 times less concentrated than the original. An easy way to perform this is to take say 5 mL of your original acid using a pipette, transfer it to a 100 mL volumetric flask and then fill to the mark with distilled or RO water.

What is a 1000x stock solution? ›

1000x concentration just means your stock is 1000x more concentrated than your working concentration. For example, if your stock is 50 mg/mL the working concentration is 50 ug/mL. To go from one to the other you dilute your stock 1000x, so for 1.5 mL you would add 1.5 uL.

How do you make a 20x stock solution? ›

So, to make 250 mL of a 20x stock solution, you would need to dissolve 2.922 g/mL x 20 = 58.44 g of NaCl in 250 mL of water. You can add the NaCl to water and stir until it is fully dissolved.

What does 50X stock solution mean? ›

The 'X' basically refers to the concentration of the solution you are working with. In other words, to get the workable concentration of 1X, you will dilute the initial stock (50X) 50 times!

What does 20X stock mean? ›

It's the price divided by earnings per share: $100 divided by five is 20x. The p/e ratio 20 (usually we denote that as 20x). This means that for every one dollar of earnings, investors are willing to pay 20 times that in value.

What does 10X and 100X mean? ›

In 10X or 100X, X is the initial money or worth or valuation or order or revenue at the time T e.g. today or this quarter or this year. And 100X is 100 times of today's money or worth or valuation or order or revenue in a specified time period.

How to make 10X stock solution? ›

To make 1 L of 10X TBS stock solution, dissolve 24 g Tris and 88 g NaCl in 900 mL of water and then adjust the pH to 7.6 and final volume to 1 L.

What is the point of a stock solution? ›

A stock solution is a known concentrated solution that is often times diluted to some lower concentration for laboratory use. Using a stock solution has several advantages, these include: Reduced storage space: a single stock solution can be prepared and stored rather than storing several vials of working solutions.

How to calculate a stock solution? ›

Stock Solution Dilutions
  1. C1 x V1 = C2 x V2
  2. C1 = stock concentration (beginning concentration)
  3. V1 = volume of stock required to prepare new solution.
  4. C2= concentration of new or working solution (desired concentration)
  5. V2= volume of new solution desired.
  6. Example:
  7. X = 160 mL of 5% stock solution diluted to 400 mL.

What does 1000x solution mean? ›

In Chemistry or biochemistry, this usually means that you prepare a solution of compound A (which could be anything) in a concentration that is 1000 times (1000x) higher than the intended final concentration to use in your experiment (again, this could be anything).

What does 50X solution mean? ›

The 'X' basically refers to the concentration of the solution you are working with. In other words, to get the workable concentration of 1X, you will dilute the initial stock (50X) 50 times!

What does 0.2% solution mean? ›

The definition of 0.2% NaCl solution is 0.2 g NaCl/ 100 g solution. The solution mass is mass of NaCl + mass water so the mass of water must be 99.8 g water.

What does 5X solution mean? ›

“X” refers to a concentration; it tells you how much more concentrated your stock solution is than the concentration at which you will use it (which is almost always 1X). So if we have a buffer that is labeled 5X, it means it is 5X more concentrated than the concentration you use in the reaction.

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