First of all, sorry if this is not the place for this kind of question or even if it's a silly question... Anyway...I came across these two terms: "promoters" and "transcription factor biding site (TFBS)". I get the concept of promoters and as far as I've read, TFBS are the same thing as promoters. However, since is my thesis, I cannot have this kind of doubt. So, is there any difference between those terms?
A promoter is the sequence generally located 5' of the transcription start site that controls transcription. A transcription factor is a protein that is generally involved in transcribing DNA into RNA. Often the term is used in a more restricted way to design proteins directly involved in controlling the expression of a gene. Some transcription factors bind DNA directly in the promoter region which can contain multiple transcription factor binding sites. Other transcription factor binding sites are not part of the promoter and can be located far away from the locus they control.
A promoter is the sequence generally located 5' of the transcription start site that controls transcription. A transcription factor is a protein that is generally involved in transcribing DNA into RNA.
The binding sites for transcription factors are often close to a gene's promoter. However, they can also be found in other parts of the DNA, sometimes very far away from the promoter, and still affect transcription of the gene.
The promoter region is given based on the coding strand, even though the RNA polymerase is actually binding to the template strand. Normally, the start of transcription, which is the first base to be incorporated into the RNA chain, is said to be at position + 1 and is called the transcription start site (TSS).
Transcription factors are proteins possessing domains that bind to the DNA of promoter or enhancer regions of specific genes. They also possess a domain that interacts with RNA polymerase II or other transcription factors and consequently regulates the amount of messenger RNA (mRNA) produced by the gene.
Each transcription factor recognizes and binds to a specific sequence in the DNA alphabet (A, C, G, and T) known as a consensus site. Although scientists have developed experimental techniques to identify consensus sites, transcription factors often bind to only a fraction of the consensus sites found in the genome.
The length of the promoter is gene-specific and can differ dramatically between genes. Consequently, the level of control of gene expression can also differ quite dramatically between genes. The purpose of the promoter is to bind transcription factors that control the initiation of transcription.
Transcription factors are proteins that bind to enhancer or promoter regions and interact to activate or repress the transcription of a particular gene. Most transcription factors can bind to specific DNA sequences. These proteins can be grouped together in families based on similarities in structure (Table 5.1).
Promoter plays a key part in the initiation phase of transcription. Its primary role is to signal where the transcription process begins on a DNA strand. Promoters are composed of a specific sequence of nucleotides that relays specific information to RNA polymerase enzymes, telling it where to initiate transcription.
Some transcription factors bind to a DNA promoter sequence near the transcription start site and help form the transcription initiation complex. Other transcription factors bind to regulatory sequences, such as enhancer sequences, and can either stimulate or repress transcription of the related gene.
TFs bind to their cognate sequences in enhancers and promoters, where they coalesce cofactors to increase chromatin accessibility and assembly of the transcription machinery.
Background. Gene expression is to large degree regulated by the specific binding of protein transcription factors to cis-regulatory transcription factor binding sites in gene promoter regions.
Typically, a TF binding site (TFBS) is 5 to 15 base pairs (bp) long within the promoter of its target gene and a TF protein usually can recognize a set of similar DNA sequences with varying degrees of binding affinity.
To begin transcribing a gene, RNA polymerase binds to the DNA of the gene at a region called the promoter. Basically, the promoter tells the polymerase where to "sit down" on the DNA and begin transcribing.
A transcription factor (TF) is a sequence-specific DNA-binding protein that modulates the transcription of a set of particular genes, and thus regulates gene expression in the cell. TFs have commonly been predicted by analyzing sequence hom*ology with the DNA-binding domains of TFs already characterized.
A promoter is a sequence that initiates and regulates the transcription of a gene. Protein binding sites in a promoter represent the most crucial elements and the corresponding proteins are called transcription factors (TFs). There is a large variety of TFs in the cell.
A promoter is a region of DNA where transcription of a gene is initiated. Promoters are a vital component of expression vectors because they control the binding of RNA polymerase to DNA. RNA polymerase transcribes DNA to mRNA which is ultimately translated into a functional protein.
Introduction: My name is Carlyn Walter, I am a lively, glamorous, healthy, clean, powerful, calm, combative person who loves writing and wants to share my knowledge and understanding with you.
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