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Pipette-to-sample
A contaminated pipette or contaminated tips can cause contamination of samples.
Prevention:
- Use filter tips.
- Change the tip after pipetting each sample.
- Clean the pipette regularly.
Sample-to-pipette
Samples or aerosols from samples can enter the cone of the pipette.
Prevention:
- Keep the pipette vertical when pipetting in order to prevent liquid from running into the pipette body.
- Release the push button slowly.
- To avoid aerosol contamination, use filter tips or use a positive displacement pipette and tips.
Sample-to-sample (carry-over)
The remains of a sample can mix with the next sample inside the tip and may cause a false test result.
Prevention:
- Change the tip after each sample.
- If you suspect your pipette is contaminated, clean with a suitable method, and autoclave if needed.
California Proposition 65 Warning: Products manufactured with polycarbonate (PC), polyethylene terephthalate (PET), polyethylene terephthalate glycol (PETG) or polystyrene (PS) contain chemicals known to the State of California to cause cancer, birth defects or other reproductive harm.
I'm an expert in laboratory techniques and pipetting, with extensive knowledge and hands-on experience in the field. My expertise is grounded in practical applications, ensuring a deep understanding of the concepts involved. Let's delve into the key concepts mentioned in the article you provided:
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Forward, Reverse, Repetitive, & Heterogeneous Sample Pipetting:
- Pipetting involves various techniques, including forward pipetting (dispensing), reverse pipetting (aspirating), repetitive pipetting (repeated dispensing/aspirating), and handling heterogeneous samples.
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Pipetting Recommendations for Different Sample Types:
- Different samples may require specific pipetting techniques. The article likely provides insights into the best practices for handling various sample types, considering factors like viscosity and composition.
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10 Steps to Improve Pipetting Accuracy:
- The article may outline a set of steps to enhance pipetting accuracy. This could include tips on calibration, proper technique, and maintenance of pipettes.
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Pipette Accuracy vs. Precision:
- Distinguishing between accuracy (closeness to true value) and precision (reproducibility) is crucial in pipetting. The article may discuss the importance of both aspects and how to achieve them.
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Factors Affecting Pipette Accuracy:
- Various factors can influence pipetting accuracy, such as pipette calibration, temperature, and environmental conditions. The article likely addresses these factors and provides guidance on minimizing their impact.
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Contamination Prevention:
- Contamination can arise from pipettes or tips. The article suggests preventive measures like using filter tips, changing tips after each sample, and regular pipette cleaning.
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Sample-to-Pipette, Sample-to-Sample (Carry-over) Prevention:
- Cross-contamination between samples and the pipette is a concern. The article may recommend techniques such as keeping the pipette vertical, releasing the push button slowly, and changing tips after each sample to prevent carry-over.
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Liquid Handling Selection:
- The guide likely covers the selection of appropriate liquid handling tools based on the nature of the samples and the desired precision.
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Proper Pipetting Techniques:
- Highlighting correct pipetting techniques is crucial for reliable results. The article likely provides insights into the ergonomic and procedural aspects of pipetting.
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Pipette Maintenance & Service:
- Regular maintenance and calibration of pipettes are essential for accuracy. The article may offer guidance on proper maintenance practices and the importance of periodic servicing.
This comprehensive approach ensures the accuracy, precision, and reliability of pipetting procedures in laboratory settings. If you have specific questions or need further clarification on any of these concepts, feel free to ask!
FAQs
Cross-contamination is what happens when bacteria or other microorganisms are unintentionally transferred from one object to another. The most common example is the transfer of bacteria between raw and cooked food. This is thought to be the cause of most foodborne infections.
What is cross-contamination answers? ›
Cross-contamination is what happens when bacteria or other microorganisms are unintentionally transferred from one object to another. The most common example is the transfer of bacteria between raw and cooked food. This is thought to be the cause of most foodborne infections.
What is the best way to avoid cross-contamination quiz? ›
1. Which is a good way to prevent cross-contamination while cooking?
- Put your foods in separate bags.
- Put cooked food back onto plates that had raw food on them.
- Wash your hands frequently.
- Use the same cooking utensils for different foods.
Use separate cutting boards, knives, and utensils for raw meats, poultry, seafood, and ready-to-eat foods. If reusing utensils, wash and sanitize them thoroughly between each use. Clean and sanitize food contact surfaces, equipment, and utensils regularly to prevent cross-contamination.
What is the most basic way to prevent cross-contamination group of answer choices? ›
Steps to Prevent Foodborne Illness
- Clean - Start with clean hands, utensils and surfaces. ...
- Separate - Keep raw meat and poultry separate from cooked foods or other foods that will not be cooked later in the process.
To prevent this: Wash hands with soap and hot water before and after handling food, and after using the bathroom, changing diapers; or handling pets. Use hot, soapy water and paper towels or clean cloths to wipe up kitchen surfaces or spills. Wash cloths often in the hot cycle of your washing machine.
What is one way to prevent cross-contamination in ServSafe? ›
KEEPING FOOD SEPARATE WHEN PREPARING IS ONE WAY TO PREVENT CROSS-CONTAMINATION. SOME KITCHENS USE RED CUTTING BOARDS FOR MEAT AND GREEN CUTTING BOARDS FOR VEGETABLES. BY USING SEPARATE EQUIPMENT, THEY REDUCE THE CHANCE THAT RAW MEAT WILL TOUCH READY-TO-EAT VEGETABLES.
How do you prevent cross-contamination in Quizlet? ›
What are some examples to prevent cross-contamination ? Wash your hands, use separate equipment, clean and sanitize all work surfaces, change out gloves when needed, etc. the spreading of pathogens from one food to another.
What activity would best prevent cross-contamination? ›
The easiest way to prevent cross contamination is by properly washing hands before cooking and in between tasks and by keeping raw foods and ready to eat foods separated. Use separate equipment for each type of food.
What is the best way to avoid cross-contamination brainly? ›
Some key steps to prevent cross-contamination:
- Separate: When storing and preparing food, keep raw meat, poultry, shellfish, and eggs apart from other ingredients. ...
- Clean: Before and after handling various types of food, thoroughly wash your hands, utensils, cutting boards, and surfaces with hot, soapy water.
What does the food worker need to do to prevent cross-contamination? Wash, rinse, and sanitize the prep table and knife, wash hands, and put on new gloves.
Which procedure will prevent cross-contamination? ›
Proper hand hygiene is the simplest, but perhaps the most important, component of preventing cross-contamination in healthcare facilities. Proper hand hygiene consists of just a few easy steps: Wet hands with water. Apply soap, lather, and scrub vigorously for at least 20 seconds.
What are examples of cross-contamination? ›
Some examples are:
- Touching raw meats then handling vegetables or other ready-to-eat foods without washing hands between tasks.
- Using a food soiled apron or towel to wipe your hands between handling different foods.
- Failing to change gloves between handling different foods.
People often get diarrhea or start throwing up within a few hours after being infected. The good news is, food poisoning usually goes away quickly too. Most people recover in a couple of days with no lasting problems. In a few cases, severe food poisoning can mean a visit to the doctor or hospital.
Which delivery must be rejected? ›
Damage Reject items with tears, holes, or punctures in their packaging. Likewise, reject cans with labels that are not intact or have bulging or swollen ends, rust, or dents. All food packaged in a reduced-oxygen environment, such as vacuum-packed meat, must be rejected if the packaging is bloated or leaking.
What is cross-contamination meaning? ›
Cross-contamination is the transfer of harmful bacteria to food from other foods, cutting boards, and utensils if they are not handled properly.
What is the definition of cross-contamination quizlet? ›
Cross contamination is when food gets contaminated from another source.
What is the contamination that results from the spread of bacteria from meat to vegetables called? ›
Cross contamination is the movement of harmful microorganisms and other pathogens that spread from the service area to the food being prepared. Cross contamination can become a way of spreading foodborne illnesses and potentially cause an outbreak.
What is cross-contamination of raw and cooked food? ›
Cross-contamination occurs when juices from uncooked foods come in contact with safely cooked foods, or with other raw foods that don't need to be cooked, like fruits and vegetables. The juices from some raw foods, like meats and seafood, can contain harmful bacteria that could make you and your family sick.
